› 2nti

X-ray diffraction
2.5Å resolution

Crystal structure of PCNA123 heterotrimer.

Released:
DOI: 10.2210/pdb2nti/pdb
PDB entry 2nti coloured by chain, front view.
PDB entry 2nti coloured by chain, side view.
PDB entry 2nti coloured by chain, top view.
Source organism: Saccharolobus solfataricus P2
Primary publication:
Structures of monomeric, dimeric and trimeric PCNA: PCNA-ring assembly and opening.
Hlinkova V, Xing G, Bauer J, Shin YJ, Dionne I, Rajashankar KR, Bell SD, Ling H
Acta Crystallogr D Biol Crystallogr 64 941-9 (2008)
PMID: 18703842

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
PDBe Complex ID:
PDB-CPX-157629 (preferred)
Entry contents:
3 distinct polypeptide molecules
Macromolecules (3 distinct):
DNA polymerase sliding clamp 1 Chains: A, D, G
Molecule details ›
Chains: A, D, G
Length: 249 amino acids
Theoretical weight: 27.52 KDa
Source organism: Saccharolobus solfataricus P2
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P57766 (Residues: 1-249; Coverage: 100%)
Gene names: C41_008, SSO0397, pcn1, pcnA-like
Sequence domains: Proliferating cell nuclear antigen, N-terminal domain
Structure domains: Proliferating Cell Nuclear Antigen
DNA polymerase sliding clamp 2 Chains: B, E, H
Molecule details ›
Chains: B, E, H
Length: 246 amino acids
Theoretical weight: 27.59 KDa
Source organism: Saccharolobus solfataricus P2
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q97Z84 (Residues: 1-245; Coverage: 100%)
Gene names: SSO1047, pcn2, pcnA-2
Sequence domains:
Structure domains: Proliferating Cell Nuclear Antigen
DNA polymerase sliding clamp 3 Chains: C, F, I
Molecule details ›
Chains: C, F, I
Length: 244 amino acids
Theoretical weight: 27.49 KDa
Source organism: Saccharolobus solfataricus P2
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P57765 (Residues: 1-244; Coverage: 100%)
Gene names: C41_016, SSO0405, pcn3, pcnA-1
Sequence domains:
Structure domains: Proliferating Cell Nuclear Antigen

Ligands and Environments

3 bound ligands:
Ligand BR 54 x BR
Ligand K 1 x K
Ligand 7PG 1 x 7PG
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 8-BM
Spacegroup: P21212
Unit cell:
a: 148.14Å b: 222.33Å c: 80.23Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.211 0.214 0.25
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

4 review citations

Replication clamps and clamp loaders.
Hedglin et al. (2013)
3 more

6 mentions without citation

Structures of monomeric, dimeric and trimeric PCNA: PCNA-ring assembly and opening.
Hlinkova et al. (2008)
5 more