› 2ntk

X-ray diffraction
2.03Å resolution

Crystal structure of PurO/IMP from Methanothermobacter thermoautotrophicus

Released:
DOI: 10.2210/pdb2ntk/pdb
PDB entry 2ntk coloured by chain, front view.
PDB entry 2ntk coloured by chain, side view.
PDB entry 2ntk coloured by chain, top view.
Source organism: Methanothermobacter thermautotrophicus
Primary publication:
A novel function for the N-terminal nucleophile hydrolase fold demonstrated by the structure of an archaeal inosine monophosphate cyclohydrolase.
Kang YN, Tran A, White RH, Ealick SE
Biochemistry 46 5050-62 (2007)
PMID: 17407260

Function and Biology Details

Reaction catalysed: 
IMP + H(2)O = 5-formamido-1-(5-phospho-D-ribosyl)imidazole-4-carboxamide
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-127874 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
IMP cyclohydrolase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 222 amino acids
Theoretical weight: 24.03 KDa
Source organism: Methanothermobacter thermautotrophicus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: O27099 (Residues: 1-202; Coverage: 100%)
Gene names: MTH_1020, purO
Sequence domains: IMP cyclohydrolase-like protein
Structure domains: Inosine monophosphate cyclohydrolase-like

Ligands and Environments

1 bound ligand:
Ligand IMP 4 x IMP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 8-BM
Spacegroup: P32
Unit cell:
a: 85.975Å b: 85.975Å c: 124.442Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.218 0.218 0.245
Expression system: Escherichia coli BL21

Quick links

Citations

2 review citations

Promiscuous anaerobes: new and unconventional metabolism in methanogenic archaea.
Grochowski et al. (2008)
1 more

3 mentions without citation

Divergence and convergence in enzyme evolution.
Galperin et al. (2012)
2 more