› 2ntz

X-ray diffraction
3.35Å resolution

Structure of a ParB-DNA complex reveals a double B-box interaction

Released:
DOI: 10.2210/pdb2ntz/pdb
PDB entry 2ntz coloured by chain, front view.
PDB entry 2ntz coloured by chain, side view.
PDB entry 2ntz coloured by chain, top view.
Source organism: Punavirus P1
Primary publication:
Structure of a four-way bridged ParB-DNA complex provides insight into P1 segrosome assembly.
Schumacher MA, Mansoor A, Funnell BE
J Biol Chem 282 10456-64 (2007)
PMID: 17293348

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
hetero hexamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115688 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
ParB/Sulfiredoxin domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 192 amino acids
Theoretical weight: 22.23 KDa
Source organism: Escherichia virus P1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q38420 (Residues: 142-333; Coverage: 58%)
Gene name: parB
Sequence domains: ParB family
5'-D(*CP*GP*TP*GP*AP*AP*AP*TP*CP*GP*CP*CP*AP*CP*GP*A)-3' Chains: E, Y
Molecule details ›
Chains: E, Y
Length: 16 nucleotides
Theoretical weight: 4.89 KDa
Source organism: Punavirus P1
Expression system: Not provided
5'-D(*TP*CP*GP*TP*GP*GP*CP*GP*AP*TP*TP*TP*CP*AP*CP*G)-3' Chains: U, W
Molecule details ›
Chains: U, W
Length: 16 nucleotides
Theoretical weight: 4.91 KDa
Source organism: Punavirus P1
Expression system: Not provided

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 12 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.1
Spacegroup: P3121
Unit cell:
a: 144.42Å b: 144.42Å c: 78.88Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.29 0.288 0.336
Expression systems:
  • Escherichia coli BL21(DE3)
  • Not provided

Quick links

Citations

6 review citations

The ParMRC system: molecular mechanisms of plasmid segregation by actin-like filaments.
Salje et al. (2010)
5 more

1 mention without citation

The structural basis for dynamic DNA binding and bridging interactions which condense the bacterial centromere.
Fisher et al. (2017)