› 2nv4

X-ray diffraction
2.2Å resolution

Crystal structure of UPF0066 protein AF0241 in complex with S-adenosylmethionine. Northeast Structural Genomics Consortium target GR27

Released:
DOI: 10.2210/pdb2nv4/pdb
PDB entry 2nv4 coloured by chain, front view.
PDB entry 2nv4 coloured by chain, side view.
PDB entry 2nv4 coloured by chain, top view.
Source organism: Archaeoglobus fulgidus
Primary publication:
Functional insights from structural genomics.
Forouhar F, Kuzin A, Seetharaman J, Lee I, Zhou W, Abashidze M, Chen Y, Yong W, Janjua H, Fang Y, Wang D, Cunningham K, Xiao R, Acton TB, Pichersky E, Klessig DF, Porter CW, Montelione GT, Tong L
J Struct Funct Genomics 8 37-44 (2007)
PMID: 17588214

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128203 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
S-adenosyl-L-methionine-binding protein AF_0241 Chains: A, B
Molecule details ›
Chains: A, B
Length: 147 amino acids
Theoretical weight: 17 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli
UniProt:
  • Canonical: O29998 (Residues: 1-139; Coverage: 100%)
Gene name: AF_0241
Sequence domains: tRNA-methyltransferase O
Structure domains: YaeB-like

Ligands and Environments

2 bound ligands:
Ligand ACT 1 x ACT
Ligand SAM 2 x SAM
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P212121
Unit cell:
a: 48.812Å b: 73.496Å c: 78.545Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.201 0.201 0.25
Expression system: Escherichia coli

Quick links

Citations

8 review citations

Ethanolamine utilization in bacterial pathogens: roles and regulation.
Garsin DA. (2010)
7 more

2 mentions without citation

Methyltransferases: Functions and Applications.
Abdelraheem et al. (2022)
1 more