› 2nvw

X-ray diffraction
2.1Å resolution

Crystal sctucture of transcriptional regulator Gal80p from kluyveromymes lactis

Released:
DOI: 10.2210/pdb2nvw/pdb
PDB entry 2nvw coloured by chain, front view.
PDB entry 2nvw coloured by chain, side view.
PDB entry 2nvw coloured by chain, top view.
Source organism: Kluyveromyces lactis
Primary publication:
Understanding a transcriptional paradigm at the molecular level. The structure of yeast Gal80p.
Thoden JB, Sellick CA, Reece RJ, Holden HM
J Biol Chem 282 1534-8 (2007)
PMID: 17121853

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo tetramer
Assembly name:
PDBe Complex ID:
PDB-CPX-170341 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Galactose/lactose metabolism regulatory protein GAL80 Chains: A, B
Molecule details ›
Chains: A, B
Length: 479 amino acids
Theoretical weight: 53.64 KDa
Source organism: Kluyveromyces lactis
Expression system: Escherichia coli
UniProt:
  • Canonical: Q06433 (Residues: 2-457; Coverage: 100%)
Gene names: GAL80, KLLA0A08162g
Sequence domains: Oxidoreductase family, NAD-binding Rossmann fold
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P21212
Unit cell:
a: 112.2Å b: 137.1Å c: 72.9Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.197 0.195 0.245
Expression system: Escherichia coli

Quick links

Citations

4 review citations

Transcriptional regulation in Saccharomyces cerevisiae: transcription factor regulation and function, mechanisms of initiation, and roles of activators and coactivators.
Hahn et al. (2011)
3 more

1 mention without citation

Structural and functional features of the NAD(P) dependent Gfo/Idh/MocA protein family oxidoreductases.
Taberman et al. (2016)