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X-ray diffraction
2.7Å resolution

X-ray Crystal Structure of Protein ytmB from Bacillus subtilis. Northeast Structural Genomics Consortium Target SR466

Released:
DOI: 10.2210/pdb2nwa/pdb
PDB entry 2nwa coloured by chain, front view.
PDB entry 2nwa coloured by chain, side view.
PDB entry 2nwa coloured by chain, top view.
Source organism: Bacillus subtilis
Entry authors: Zhou W, Forouhar F, Seetharaman J, Wang D, Cunningham K, Ma L-C, Fang Y, Xiao R, Baran MC, Acton TB, Montelione GT, Hunt JF, Tong L, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
homo tetramer (preferred)
homo octamer
Assembly name:
PDBe Complex ID:
PDB-CPX-128533 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hypothetical protein ytmB Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 88 amino acids
Theoretical weight: 10.54 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: O34365 (Residues: 1-80; Coverage: 100%)
Gene names: BSU30570, ytmB
Sequence domains: Protein of unknown function (DUF2584)
Structure domains: Hypothetical PUA domain-like; domain 1

Ligands and Environments

1 bound ligand:
Ligand SO4 8 x SO4
1 modified residue:
Ligand MSE 32 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P21
Unit cell:
a: 60.662Å b: 110.739Å c: 60.672Å
α: 90° β: 111.06° γ: 90°
R-values:
R R work R free
0.271 0.266 0.285
Expression system: Escherichia coli

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