› 2nwl

X-ray diffraction
2.96Å resolution

Crystal structure of GltPh in complex with L-Asp

Released:
DOI: 10.2210/pdb2nwl/pdb
PDB entry 2nwl coloured by chain, front view.
PDB entry 2nwl coloured by chain, side view.
PDB entry 2nwl coloured by chain, top view.
Source organism: Pyrococcus horikoshii
Primary publication:
Coupling substrate and ion binding to extracellular gate of a sodium-dependent aspartate transporter.
Boudker O, Ryan RM, Yernool D, Shimamoto K, Gouaux E
Nature 445 387-93 (2007)
PMID: 17230192

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129794 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate transporter homolog Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 422 amino acids
Theoretical weight: 44.63 KDa
Source organism: Pyrococcus horikoshii
Expression system: Escherichia coli
UniProt:
  • Canonical: O59010 (Residues: 1-417; Coverage: 98%)
Gene name: PH1295
Sequence domains: Sodium:dicarboxylate symporter family
Structure domains: Sodium:dicarboxylate symporter

Ligands and Environments

2 bound ligands:
Ligand PLM 3 x PLM
Ligand ASP 3 x ASP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P61
Unit cell:
a: 115.296Å b: 115.296Å c: 323.781Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.238 0.236 0.265
Expression system: Escherichia coli

Quick links

Citations

56 review citations

Glutamate transporters: confining runaway excitation by shaping synaptic transmission.
Tzingounis et al. (2007)
55 more

26 mentions without citation

Elevator-type mechanisms of membrane transport.
Garaeva et al. (2020)
25 more