› 2nww

X-ray diffraction
3.2Å resolution

Crystal structure of GltPh in complex with TBOA

Released:
DOI: 10.2210/pdb2nww/pdb
PDB entry 2nww coloured by chain, front view.
PDB entry 2nww coloured by chain, side view.
PDB entry 2nww coloured by chain, top view.
Source organism: Pyrococcus horikoshii
Primary publication:
Coupling substrate and ion binding to extracellular gate of a sodium-dependent aspartate transporter.
Boudker O, Ryan RM, Yernool D, Shimamoto K, Gouaux E
Nature 445 387-93 (2007)
PMID: 17230192

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-129794 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamate transporter homolog Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 422 amino acids
Theoretical weight: 44.64 KDa
Source organism: Pyrococcus horikoshii
Expression system: Escherichia coli
UniProt:
  • Canonical: O59010 (Residues: 1-417; Coverage: 98%)
Gene name: PH1295
Sequence domains: Sodium:dicarboxylate symporter family
Structure domains: Sodium:dicarboxylate symporter

Ligands and Environments

1 bound ligand:
Ligand TB1 3 x TB1
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ALS BEAMLINE 8.2.2
Spacegroup: P61
Unit cell:
a: 115.245Å b: 115.245Å c: 322.372Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.242 0.241 0.26
Expression system: Escherichia coli

Quick links

Citations

50 review citations

Glutamate transporters: confining runaway excitation by shaping synaptic transmission.
Tzingounis et al. (2007)
49 more

45 mentions without citation

Unlocking the molecular secrets of sodium-coupled transporters.
Krishnamurthy et al. (2009)
44 more