› 2nxq

X-ray diffraction
2.4Å resolution

Crystal structure of calcium binding protein 1 from Entamoeba histolytica: a novel arrangement of EF hand motifs

Released:
DOI: 10.2210/pdb2nxq/pdb
PDB entry 2nxq coloured by chain, front view.
PDB entry 2nxq coloured by chain, side view.
PDB entry 2nxq coloured by chain, top view.
Source organism: Entamoeba histolytica HM-1:IMSS
Primary publication:
Crystal structure of calcium binding protein-1 from Entamoeba histolytica: a novel arrangement of EF hand motifs.
Kumar S, Padhan N, Alam N, Gourinath S
Proteins 68 990-8 (2007)
PMID: 17554780

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
homo trimer
homo hexamer
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-153663 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calcium-binding protein 1 Chains: A, B
Molecule details ›
Chains: A, B
Length: 134 amino acids
Theoretical weight: 14.97 KDa
Source organism: Entamoeba histolytica HM-1:IMSS
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P38505 (Residues: 1-134; Coverage: 100%)
Gene names: CaBP1, EHI_120900
Sequence domains: EF-hand domain pair
Structure domains: EF-hand

Ligands and Environments

2 bound ligands:
Ligand CA 4 x CA
Ligand ACT 5 x ACT
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE A1
Spacegroup: P63
Unit cell:
a: 95.25Å b: 95.25Å c: 64.996Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.258 0.258 0.282
Expression system: Escherichia coli BL21

Quick links

Citations

1 review citation

Entamoeba histolytica and pathogenesis: A calcium connection.
Babuta et al. (2020)
7 other articles

5 mentions without citation

Structural and functional diversity of Entamoeba histolytica calcium-binding proteins.
Kumar et al. (2020)
4 more