› 2nz0

X-ray diffraction
3.2Å resolution

Crystal structure of potassium channel Kv4.3 in complex with its regulatory subunit KChIP1

Released:
DOI: 10.2210/pdb2nz0/pdb
PDB entry 2nz0 coloured by chain, front view.
PDB entry 2nz0 coloured by chain, side view.
PDB entry 2nz0 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural basis for modulation of Kv4 K+ channels by auxiliary KChIP subunits.
Wang H, Yan Y, Liu Q, Huang Y, Shen Y, Chen L, Chen Y, Yang Q, Hao Q, Wang K, Chai J
Nat Neurosci 10 32-9 (2007)
PMID: 17187064

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assemblies composition:
hetero octamer (preferred)
hetero hexadecamer
Assembly name:
PDBe Complex ID:
PDB-CPX-192747 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Kv channel-interacting protein 1 Chains: A, C
Molecule details ›
Chains: A, C
Length: 180 amino acids
Theoretical weight: 21.15 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9NZI2 (Residues: 49-227; Coverage: 79%)
Gene names: KCHIP1, KCNIP1, VABP
Sequence domains:
Structure domains: EF-hand
Potassium voltage-gated channel subfamily D member 3 Chains: B, D
Molecule details ›
Chains: B, D
Length: 140 amino acids
Theoretical weight: 16.58 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9UK17 (Residues: 6-145; Coverage: 21%)
Gene name: KCND3
Sequence domains:
Structure domains: Potassium Channel Kv1.1; Chain A

Ligands and Environments

2 bound ligands:
Ligand CA 4 x CA
Ligand ZN 2 x ZN
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CHESS BEAMLINE F1
Spacegroup: I222
Unit cell:
a: 112.186Å b: 112.213Å c: 141.051Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.265 0.261 0.31
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

24 review citations

Ancillary subunits associated with voltage-dependent K+ channels.
Pongs et al. (2010)
23 more

5 mentions without citation

Co-assembly of Kv4 {alpha} subunits with K+ channel-interacting protein 2 stabilizes protein expression and promotes surface retention of channel complexes.
Foeger et al. (2010)
4 more