› 2o18

X-ray diffraction
2.2Å resolution

Crystal structure of a Thiamine biosynthesis lipoprotein apbE, NorthEast Strcutural Genomics target ER559

Released:
DOI: 10.2210/pdb2o18/pdb
PDB entry 2o18 coloured by chain, front view.
PDB entry 2o18 coloured by chain, side view.
PDB entry 2o18 coloured by chain, top view.
Source organism: Escherichia coli
Entry authors: Seetharaman J, Su M, Wang D, Fang Y, Cunningham K, Ma L, Xiao R, Liu J, Baran MC, Acton TB, Rost B, Montelione GT, Hunt JF, Tong L, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Reaction catalysed: 
FAD + [protein]-L-threonine = [protein]-FMN-L-threonine + AMP
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142025 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
FAD:protein FMN transferase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 340 amino acids
Theoretical weight: 38.17 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli
UniProt:
  • Canonical: P0AB85 (Residues: 21-351; Coverage: 100%)
Gene names: JW5368, apbE, b2214, yojK, yojL
Sequence domains: ApbE family
Structure domains: ApbE-like domains

Ligands and Environments

1 bound ligand:
Ligand CA 4 x CA
1 modified residue:
Ligand MSE 44 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X12C
Spacegroup: P1
Unit cell:
a: 57.32Å b: 70.653Å c: 86.637Å
α: 75.76° β: 71.67° γ: 69.46°
R-values:
R R work R free
0.233 0.233 0.284
Expression system: Escherichia coli

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