› 2o1e

X-ray diffraction
2.6Å resolution

Crystal Structure of the Metal-dependent Lipoprotein YcdH from Bacillus subtilis, Northeast Structural Genomics Target SR583

Released:
DOI: 10.2210/pdb2o1e/pdb
PDB entry 2o1e coloured by chain, front view.
PDB entry 2o1e coloured by chain, side view.
PDB entry 2o1e coloured by chain, top view.
Source organism: Bacillus subtilis
Entry authors: Forouhar F, Zhou W, Seetharaman J, Chen CX, Fang Y, Cunningham K, Ma L-C, Janjua H, Xiao R, Baran MC, Liu J, Acton TB, Montelione GT, Hunt JF, Tong L, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128629 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
High-affinity zinc uptake system protein ZnuA Chains: A, B
Molecule details ›
Chains: A, B
Length: 312 amino acids
Theoretical weight: 35.46 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: O34966 (Residues: 22-319; Coverage: 100%)
Gene names: BSU02850, adcA, ycdH, znuA
Sequence domains: Zinc-uptake complex component A periplasmic
Structure domains: Nitrogenase molybdenum iron protein domain

Ligands and Environments

1 bound ligand:
Ligand MN 2 x MN
1 modified residue:
Ligand MSE 16 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4C
Spacegroup: P1
Unit cell:
a: 45.292Å b: 54.344Å c: 62.84Å
α: 103.7° β: 100.46° γ: 96.75°
R-values:
R R work R free
0.223 0.223 0.29
Expression system: Escherichia coli

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