› 2o1j

X-ray diffraction
2.7Å resolution

Structure of the extended diarrhea-inducing domain of rotavirus enterotoxigenic protein NSP4

Released:
DOI: 10.2210/pdb2o1j/pdb
PDB entry 2o1j coloured by chain, front view.
PDB entry 2o1j coloured by chain, side view.
PDB entry 2o1j coloured by chain, top view.
Source organism: Rotavirus str. I321
Entry authors: Suguna K, Durga Rao C, Deepa R

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192955 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Nonstructural protein NSP4 Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 52 amino acids
Theoretical weight: 6.36 KDa
Source organism: Rotavirus str. I321
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9PYC1 (Residues: 95-146; Coverage: 30%)
Structure domains: Single alpha-helices involved in coiled-coils or other helix-helix interfaces

Ligands and Environments

1 bound ligand:
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: C2221
Unit cell:
a: 48.614Å b: 180.432Å c: 48.691Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.251 0.248 0.314
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Viroporins: structure, function and potential as antiviral targets.
Scott et al. (2015)
1 more

4 mentions without citation

Viral calciomics: interplays between Ca2+ and virus.
Zhou et al. (2009)
3 more