› 2o1m

X-ray diffraction
2Å resolution

Crystal structure of the probable amino-acid ABC transporter extracellular-binding protein ytmK from Bacillus subtilis. Northeast Structural Genomics Consortium target SR572

Released:
DOI: 10.2210/pdb2o1m/pdb
PDB entry 2o1m coloured by chain, front view.
PDB entry 2o1m coloured by chain, side view.
PDB entry 2o1m coloured by chain, top view.
Source organism: Bacillus subtilis
Entry authors: Kuzin AP, Su M, Seetharaman J, Chen CX, Fang Y, Cunningham K, Ma LC, Xiao R, Liu J, Baran MC, Acton TB, Rost B, Montelione G, Tong L, Hunt JF, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128613 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-cystine-binding protein TcyK Chains: A, B
Molecule details ›
Chains: A, B
Length: 258 amino acids
Theoretical weight: 29.4 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli
UniProt:
  • Canonical: O34852 (Residues: 22-270; Coverage: 100%)
Gene names: BSU29370, tcyK, ytmK
Sequence domains: Bacterial extracellular solute-binding proteins, family 3
Structure domains: Periplasmic binding protein-like II

Ligands and Environments

1 bound ligand:
Ligand SO4 4 x SO4
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P21212
Unit cell:
a: 81.563Å b: 88.931Å c: 76.697Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.23 0.23 0.264
Expression system: Escherichia coli

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