› 2o2d

X-ray diffraction
1.9Å resolution

Crystal structure of phosphoglucose isomerase from Trypanosoma brucei complexed with citrate

Released:
DOI: 10.2210/pdb2o2d/pdb
PDB entry 2o2d coloured by chain, front view.
PDB entry 2o2d coloured by chain, side view.
PDB entry 2o2d coloured by chain, top view.
Source organism: Trypanosoma brucei brucei
Primary publication:
Crystal structure of phosphoglucose isomerase from Trypanosoma brucei complexed with glucose-6-phosphate at 1.6 A resolution.
Arsenieva D, Appavu BL, Mazock GH, Jeffery CJ
Proteins 74 72-80 (2009)
PMID: 18561188

Function and Biology Details

Reaction catalysed: 
Alpha-D-glucose 6-phosphate = beta-D-fructofuranose 6-phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-146553 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glucose-6-phosphate isomerase, glycosomal Chains: A, B, C
Molecule details ›
Chains: A, B, C
Length: 613 amino acids
Theoretical weight: 68.46 KDa
Source organism: Trypanosoma brucei brucei
Expression system: Escherichia coli
UniProt:
  • Canonical: P13377 (Residues: 1-607; Coverage: 100%)
Gene name: PGI
Sequence domains: Phosphoglucose isomerase
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CIT 3 x CIT
Ligand GOL 3 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: C2221
Unit cell:
a: 124.203Å b: 217.391Å c: 126.433Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.213 0.213 0.236
Expression system: Escherichia coli

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Citations

1 review citation

State of the art in African trypanosome drug discovery.
Jacobs et al. (2011)
3 other articles

3 mentions without citation

The Potential of Secondary Metabolites from Plants as Drugs or Leads against Protozoan Neglected Diseases-Part III: In-Silico Molecular Docking Investigations.
Ogungbe et al. (2016)
2 more