› 2o2x

X-ray diffraction
1.5Å resolution

Crystal structure of a putative had-like phosphatase (mll2559) from mesorhizobium loti at 1.50 A resolution

Released:
DOI: 10.2210/pdb2o2x/pdb
PDB entry 2o2x coloured by chain, front view.
PDB entry 2o2x coloured by chain, side view.
PDB entry 2o2x coloured by chain, top view.
Source organism: Mesorhizobium loti
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Reaction catalysed: 
D-glycero-beta-D-manno-heptose 1,7-bisphosphate + H(2)O = D-glycero-beta-D-manno-heptose 1-phosphate + phosphate
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189154 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
D-glycero-beta-D-manno-heptose-1,7-bisphosphate 7-phosphatase Chain: A
Molecule details ›
Chain: A
Length: 218 amino acids
Theoretical weight: 23.56 KDa
Source organism: Mesorhizobium loti
Expression system: Escherichia coli
UniProt:
  • Canonical: Q98I56 (Residues: 1-217; Coverage: 100%)
Gene names: gmhB, mll2559
Sequence domains: HAD-hyrolase-like
Structure domains: HAD superfamily/HAD-like

Ligands and Environments

1 bound ligand:
Ligand GOL 2 x GOL
1 modified residue:
Ligand MSE 6 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P21
Unit cell:
a: 36.48Å b: 66.27Å c: 41.02Å
α: 90° β: 108.53° γ: 90°
R-values:
R R work R free
0.139 0.138 0.167
Expression system: Escherichia coli

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