› 2o3a

X-ray diffraction
2.2Å resolution

Crystal structure of a protein AF_0751 from Archaeoglobus fulgidus

Released:
DOI: 10.2210/pdb2o3a/pdb
PDB entry 2o3a coloured by chain, front view.
PDB entry 2o3a coloured by chain, side view.
PDB entry 2o3a coloured by chain, top view.
Source organism: Archaeoglobus fulgidus
Entry authors: Bonanno JB, Dickey M, Bain KT, Wu B, Slocombe A, Sridhar V, Sauder JM, Burley SK, Almo SC, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
S-adenosyl-L-methionine + cytidine(56) in tRNA = S-adenosyl-L-homocysteine + 2'-O-methylcytidine(56) in tRNA
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128139 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
tRNA (cytidine(56)-2'-O)-methyltransferase Chains: A, B
Molecule details ›
Chains: A, B
Length: 178 amino acids
Theoretical weight: 20.27 KDa
Source organism: Archaeoglobus fulgidus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O29507 (Residues: 2-168; Coverage: 99%)
Gene name: AF_0751
Sequence domains: tRNA ribose 2'-O-methyltransferase, aTrm56
Structure domains: Alpha/beta knot

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P212121
Unit cell:
a: 53.294Å b: 55.89Å c: 108.784Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.233 0.23 0.292
Expression system: Escherichia coli BL21(DE3)

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