› 2o3y

X-ray diffraction
2.7Å resolution

Crystal Structure of the Homo sapiens Cytoplasmic Ribosomal Decoding Site in Presence of Paromamine Derivative NB30

Released:
DOI: 10.2210/pdb2o3y/pdb
PDB entry 2o3y coloured by chain, front view.
PDB entry 2o3y coloured by chain, side view.
PDB entry 2o3y coloured by chain, top view.
Source organism: Synthetic construct
Primary publication:
Differential selectivity of natural and synthetic aminoglycosides towards the eukaryotic and prokaryotic decoding A sites.
Kondo J, Hainrichson M, Nudelman I, Shallom-Shezifi D, Barbieri CM, Pilch DS, Westhof E, Baasov T
Chembiochem 8 1700-9 (2007)
PMID: 17705310

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-196544 (preferred)
Entry contents:
1 distinct RNA molecule
Macromolecule:
RNA (5'-R(*UP*UP*GP*CP*GP*UP*CP*GP*CP*UP*CP*CP*GP*GP*AP*AP*AP*AP*GP*UP*CP*GP*C)-3') Chains: A, B
Molecule details ›
Chains: A, B
Length: 23 nucleotides
Theoretical weight: 7.36 KDa
Source organism: Synthetic construct
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand SPM 1 x SPM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID23-2
Spacegroup: P21212
Unit cell:
a: 47.94Å b: 49.23Å c: 57.46Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.245 0.245 0.282
Expression system: Not provided

Quick links

Citations

6 review citations

The future of aminoglycosides: the end or renaissance?
Houghton et al. (2010)
5 more