› 2o5a

X-ray diffraction
2.7Å resolution

Crystal structure of Q9KD89 from Bacillus halodurans. Northeast Structural Genomics target BhR21

Released:
DOI: 10.2210/pdb2o5a/pdb
PDB entry 2o5a coloured by chain, front view.
PDB entry 2o5a coloured by chain, side view.
PDB entry 2o5a coloured by chain, top view.
Source organism: Alkalihalobacillus halodurans
Entry authors: Benach J, Su M, Seetharaman J, Ho CH, Janjua H, Cunningham K, Ma L-C, Xiao R, Liu J, Baran MC, Acton TB, Rost B, Montelione GT, Hunt JF, Tong L, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-191842 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ribosomal silencing factor RsfS Chains: A, B
Molecule details ›
Chains: A, B
Length: 125 amino acids
Theoretical weight: 14.38 KDa
Source organism: Alkalihalobacillus halodurans
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9KD89 (Residues: 1-117; Coverage: 100%)
Gene names: BH1328, rsfS
Sequence domains: Ribosomal silencing factor during starvation
Structure domains: Beta Polymerase, domain 2

Ligands and Environments

2 bound ligands:
Ligand MN 4 x MN
Ligand SO4 1 x SO4
1 modified residue:
Ligand MSE 4 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: P21212
Unit cell:
a: 119.936Å b: 32.021Å c: 59.546Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.251 0.251 0.278
Expression system: Escherichia coli

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