› 2o6p

X-ray diffraction
1.5Å resolution

Crystal Structure of the heme-IsdC complex

Released:
DOI: 10.2210/pdb2o6p/pdb
PDB entry 2o6p coloured by chain, front view.
PDB entry 2o6p coloured by chain, side view.
PDB entry 2o6p coloured by chain, top view.
Source organism: Staphylococcus aureus subsp. aureus N315
Primary publication:
Crystal structure of the heme-IsdC complex, the central conduit of the Isd iron/heme uptake system in Staphylococcus aureus.
Sharp KH, Schneider S, Cockayne A, Paoli M
J Biol Chem 282 10625-31 (2007)
PMID: 17287214

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-185203 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Iron-regulated surface determinant protein C Chains: A, B
Molecule details ›
Chains: A, B
Length: 161 amino acids
Theoretical weight: 17.81 KDa
Source organism: Staphylococcus aureus subsp. aureus N315
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: Q8KQR1 (Residues: 30-188; Coverage: 80%)
Gene names: SAOUHSC_01082, isdC, sirD
Sequence domains: Iron Transport-associated domain
Structure domains: Immunoglobulin-like

Ligands and Environments

3 bound ligands:
Ligand ZN 6 x ZN
Ligand CL 2 x CL
Ligand HEM 2 x HEM
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: ESRF BEAMLINE ID29
Spacegroup: P212121
Unit cell:
a: 47.489Å b: 70.441Å c: 88.685Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.195 0.193 0.222
Expression system: Escherichia coli BL21

Quick links

Citations

11 review citations

Molecular mechanisms of Staphylococcus aureus iron acquisition.
Hammer et al. (2011)
10 more

14 mentions without citation

Iron Metabolism at the Interface between Host and Pathogen: From Nutritional Immunity to Antibacterial Development.
Marchetti et al. (2020)
13 more