› 2o7r

X-ray diffraction
1.4Å resolution

Plant carboxylesterase AeCXE1 from Actinidia eriantha with acyl adduct

Released:
DOI: 10.2210/pdb2o7r/pdb
PDB entry 2o7r coloured by chain, front view.
PDB entry 2o7r coloured by chain, side view.
PDB entry 2o7r coloured by chain, top view.
Source organism: Actinidia eriantha
Primary publication:
High-resolution crystal structure of plant carboxylesterase AeCXE1, from Actinidia eriantha, and its complex with a high-affinity inhibitor paraoxon.
Ileperuma NR, Marshall SD, Squire CJ, Baker HM, Oakeshott JG, Russell RJ, Plummer KM, Newcomb RD, Baker EN
Biochemistry 46 1851-9 (2007)
PMID: 17256879

Function and Biology Details

Reaction catalysed: 
A carboxylic ester + H(2)O = an alcohol + a carboxylate
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171208 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Carboxylesterase 1 Chain: A
Molecule details ›
Chain: A
Length: 338 amino acids
Theoretical weight: 37.45 KDa
Source organism: Actinidia eriantha
Expression system: Escherichia coli
UniProt:
  • Canonical: Q0ZPV7 (Residues: 1-335; Coverage: 100%)
Gene name: CXE1
Sequence domains: alpha/beta hydrolase fold
Structure domains: alpha/beta hydrolase

Ligands and Environments

1 bound ligand:
Ligand 4PA 1 x 4PA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL9-1
Spacegroup: C2
Unit cell:
a: 156.565Å b: 53.82Å c: 42.328Å
α: 90° β: 102.48° γ: 90°
R-values:
R R work R free
0.2 0.199 0.218
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Biochemistry of Apple Aroma: A Review.
Espino-Díaz et al. (2016)
1 more

5 mentions without citation

Plant pectin acetylesterase structure and function: new insights from bioinformatic analysis.
Philippe et al. (2017)
4 more