› 2o8l

X-ray diffraction
1.5Å resolution

Structure of V8 protease from staphylococcus aureus

Released:
DOI: 10.2210/pdb2o8l/pdb
PDB entry 2o8l coloured by chain, front view.
PDB entry 2o8l coloured by chain, side view.
PDB entry 2o8l coloured by chain, top view.
Source organism: Staphylococcus aureus
Primary publication:
The structure of a universally employed enzyme: V8 protease from Staphylococcus aureus.
Prasad L, Leduc Y, Hayakawa K, Delbaere LT
Acta Crystallogr D Biol Crystallogr 60 256-9 (2004)
PMID: 14747701

Function and Biology Details

Reaction catalysed: 
Preferential cleavage: Glu-|-, Asp-|-.
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-189401 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Glutamyl endopeptidase Chain: A
Molecule details ›
Chain: A
Length: 274 amino acids
Theoretical weight: 29.68 KDa
Source organism: Staphylococcus aureus
UniProt:
  • Canonical: Q99V45 (Residues: 69-342; Coverage: 88%)
Gene names: SAV1048, sspA
Sequence domains: Trypsin-like peptidase domain
Structure domains: Trypsin-like serine proteases

Ligands and Environments

1 bound ligand:
Ligand K 1 x K
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: CLSI BEAMLINE 08ID-1
Spacegroup: P6522
Unit cell:
a: 59.43Å b: 59.43Å c: 214.01Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.19 0.188 0.219

Quick links

Citations

4 review citations

Staphylococcus aureus Secreted Toxins and Extracellular Enzymes.
Tam et al. (2019)
3 more

7 mentions without citation

Exfoliative toxins of Staphylococcus aureus.
Bukowski et al. (2010)
6 more