› 2o9b

X-ray diffraction
2.15Å resolution

Crystal Structure of Bacteriophytochrome chromophore binding domain

Released:
DOI: 10.2210/pdb2o9b/pdb
PDB entry 2o9b coloured by chain, front view.
PDB entry 2o9b coloured by chain, side view.
PDB entry 2o9b coloured by chain, top view.
Source organism: Deinococcus radiodurans
Primary publication:
High resolution structure of Deinococcus bacteriophytochrome yields new insights into phytochrome architecture and evolution.
Wagner JR, Zhang J, Brunzelle JS, Vierstra RD, Forest KT
J Biol Chem 282 12298-309 (2007)
PMID: 17322301

Function and Biology Details

Reaction catalysed: 
ATP + protein L-histidine = ADP + protein N-phospho-L-histidine
Biochemical function:
  • not assigned
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-193367 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bacteriophytochrome Chain: A
Molecule details ›
Chain: A
Length: 342 amino acids
Theoretical weight: 37.02 KDa
Source organism: Deinococcus radiodurans
Expression system: Escherichia coli
UniProt:
  • Canonical: Q9RZA4 (Residues: 1-321; Coverage: 43%)
Gene names: DR_A0050, bphP
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand LBV 1 x LBV
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BRUKER AXS MICROSTAR
Spacegroup: C2
Unit cell:
a: 89.5Å b: 51.5Å c: 80.8Å
α: 90° β: 116.4° γ: 90°
R-values:
R R work R free
0.191 0.189 0.237
Expression system: Escherichia coli

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Citations

22 review citations

Decoding of light signals by plant phytochromes and their interacting proteins.
Bae et al. (2008)
21 more

10 mentions without citation

Probing designability via a generalized model of helical bundle geometry.
Grigoryan et al. (2011)
9 more