› 2oa5

X-ray diffraction
2.1Å resolution

Crystal structure of ORF52 from Murid herpesvirus (MUHV-4) (Murine gammaherpesvirus 68) at 2.1 A resolution. Northeast Structural Genomics Consortium target MHR28B.

Released:
DOI: 10.2210/pdb2oa5/pdb
PDB entry 2oa5 coloured by chain, front view.
PDB entry 2oa5 coloured by chain, side view.
PDB entry 2oa5 coloured by chain, top view.
Source organism: Murid gammaherpesvirus 4
Primary publication:
Structural and functional studies of the abundant tegument protein ORF52 from murine gammaherpesvirus 68.
Benach J, Wang L, Chen Y, Ho CK, Lee S, Seetharaman J, Xiao R, Acton TB, Montelione GT, Deng H, Sun R, Tong L
J Biol Chem 282 31534-41 (2007)
PMID: 17699518

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domain:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-161018 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
52 protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 110 amino acids
Theoretical weight: 12.52 KDa
Source organism: Murid gammaherpesvirus 4
Expression system: Escherichia coli
UniProt:
  • Canonical: P88989 (Residues: 1-102; Coverage: 76%)
Gene names: 52, BQLF2, GAMMAHV.ORF52
Sequence domains: Herpesvirus BLRF2 protein
Structure domains: YejL-like

Ligands and Environments

1 bound ligand:
Ligand PE5 2 x PE5
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X4A
Spacegroup: C2
Unit cell:
a: 93.462Å b: 49.393Å c: 56.379Å
α: 90° β: 111.88° γ: 90°
R-values:
R R work R free
0.215 0.215 0.241
Expression system: Escherichia coli

Quick links

Citations

2 review citations

Role of tegument proteins in herpesvirus assembly and egress.
Guo et al. (2010)
1 more

3 mentions without citation

VP22 core domain from Herpes simplex virus 1 reveals a surprising structural conservation in both the Alpha- and Gammaherpesvirinae subfamilies.
Hew et al. (2015)
2 more