› 2oaa

X-ray diffraction
1.5Å resolution

Restriction endonuclease MvaI-cognate DNA substrate complex

Released:
DOI: 10.2210/pdb2oaa/pdb
PDB entry 2oaa coloured by chain, front view.
PDB entry 2oaa coloured by chain, side view.
PDB entry 2oaa coloured by chain, top view.
Source organism: Kocuria varians
Primary publication:
Restriction endonuclease MvaI is a monomer that recognizes its target sequence asymmetrically.
Kaus-Drobek M, Czapinska H, SokoĊ‚owska M, Tamulaitis G, Szczepanowski RH, Urbanke C, Siksnys V, Bochtler M
Nucleic Acids Res 35 2035-46 (2007)
PMID: 17344322

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115726 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
MvaI/BcnI restriction endonuclease domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 249 amino acids
Theoretical weight: 28.7 KDa
Source organism: Kocuria varians
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8RNV5 (Residues: 1-246; Coverage: 100%)
Gene name: mvaIR
Sequence domains: MvaI/BcnI restriction endonuclease family
Structure domains:
5'-D(*GP*GP*TP*AP*CP*CP*TP*GP*GP*AP*T)-3' Chains: C, E
Molecule details ›
Chains: C, E
Length: 11 nucleotides
Theoretical weight: 3.39 KDa
Source organism: Kocuria varians
Expression system: Not provided
5'-D(*CP*AP*TP*CP*CP*AP*GP*GP*TP*AP*C)-3' Chains: D, F
Molecule details ›
Chains: D, F
Length: 11 nucleotides
Theoretical weight: 3.32 KDa
Source organism: Kocuria varians
Expression system: Not provided

Ligands and Environments

1 bound ligand:
Ligand CA 7 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: P21
Unit cell:
a: 53.848Å b: 81.38Å c: 71.308Å
α: 90° β: 90.52° γ: 90°
R-values:
R R work R free
0.177 0.176 0.194
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

23 citation in other articles

Structural and evolutionary classification of Type II restriction enzymes based on theoretical and experimental analyses.
Orlowski et al. (2008)
22 more

7 mentions without citation

Type I restriction enzymes and their relatives.
Loenen et al. (2014)
6 more