› 2ob3

X-ray diffraction
1.04Å resolution

Structure of Phosphotriesterase mutant H257Y/L303T

Released:
DOI: 10.2210/pdb2ob3/pdb
PDB entry 2ob3 coloured by chain, front view.
PDB entry 2ob3 coloured by chain, side view.
PDB entry 2ob3 coloured by chain, top view.
Source organism: Brevundimonas diminuta
Entry authors: Kim J, Ramagopal UA, Tsai P, Raushel FM, Almo SC

Function and Biology Details

Reaction catalysed: 
An aryl dialkyl phosphate + H(2)O = dialkyl phosphate + an aryl alcohol
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-141251 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Parathion hydrolase Chains: A, B
Molecule details ›
Chains: A, B
Length: 330 amino acids
Theoretical weight: 35.89 KDa
Source organism: Brevundimonas diminuta
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0A434 (Residues: 35-364; Coverage: 98%)
Gene name: opd
Sequence domains: Phosphotriesterase family
Structure domains: Metal-dependent hydrolases

Ligands and Environments

2 bound ligands:
Ligand ZN 4 x ZN
Ligand BTB 2 x BTB
1 modified residue:
Ligand KCX 2 x KCX

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P1
Unit cell:
a: 43.362Å b: 45.374Å c: 79.211Å
α: 104.86° β: 93.27° γ: 97.81°
R-values:
R R work R free
0.105 0.105 0.127
Expression system: Escherichia coli BL21(DE3)

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