› 2obg

X-ray diffraction
2.35Å resolution

Crystal Structure of Monobody MBP-74/Maltose Binding Protein Fusion Complex

Released:
DOI: 10.2210/pdb2obg/pdb
PDB entry 2obg coloured by chain, front view.
PDB entry 2obg coloured by chain, side view.
PDB entry 2obg coloured by chain, top view.
Source organism: Escherichia coli
Primary publication:
High-affinity single-domain binding proteins with a binary-code interface.
Koide A, Gilbreth RN, Esaki K, Tereshko V, Koide S
Proc Natl Acad Sci U S A 104 6632-7 (2007)
PMID: 17420456

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-142637 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Maltose/maltodextrin-binding periplasmic protein Chain: A
Molecule details ›
Chain: A
Length: 461 amino acids
Theoretical weight: 50.54 KDa
Source organism: Escherichia coli
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0AEX9 (Residues: 31-396; Coverage: 99%)
Gene names: JW3994, b4034, malE
Sequence domains: Bacterial extracellular solute-binding protein
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 24-ID-C
Spacegroup: P41
Unit cell:
a: 68.461Å b: 68.461Å c: 112.625Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.198 0.25
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

24 review citations

Yeast surface display for protein engineering and characterization.
Gai et al. (2007)
23 more

13 mentions without citation

The importance of being tyrosine: lessons in molecular recognition from minimalist synthetic binding proteins.
Koide et al. (2009)
12 more