› 2obk

X-ray diffraction
2.7Å resolution

X-Ray structure of the putative Se binding protein from Pseudomonas fluorescens. Northeast Structural Genomics Consortium target PlR6.

Released:
DOI: 10.2210/pdb2obk/pdb
PDB entry 2obk coloured by chain, front view.
PDB entry 2obk coloured by chain, side view.
PDB entry 2obk coloured by chain, top view.
Source organism: Pseudomonas protegens Pf-5
Entry authors: Kuzin AP, Su M, Seetharaman J, Chen CX, Fang Y, Cunningham K, Ma LC, Xiao R, Liu J, Baran MC, Acton TB, Rost B, Montelione GT, Tong L, Hunt JF, Northeast Structural Genomics Consortium (NESG)

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-175626 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
SelT/selW/selH selenoprotein domain protein Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 103 amino acids
Theoretical weight: 11.95 KDa
Source organism: Pseudomonas protegens Pf-5
Expression system: Escherichia coli
UniProt:
  • Canonical: Q4KGC5 (Residues: 1-95; Coverage: 100%)
Gene name: PFL_1582
Sequence domains: Rdx family
Structure domains: Glutaredoxin

Ligands and Environments

No bound ligands
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X3A
Spacegroup: P212121
Unit cell:
a: 54.311Å b: 112.251Å c: 148.459Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.215 0.292
Expression system: Escherichia coli

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