› 2ocd

X-ray diffraction
2.45Å resolution

Crystal structure of L-asparaginase I from Vibrio cholerae O1 biovar eltor str. N16961

Released:
DOI: 10.2210/pdb2ocd/pdb
PDB entry 2ocd coloured by chain, front view.
PDB entry 2ocd coloured by chain, side view.
PDB entry 2ocd coloured by chain, top view.
Source organism: Vibrio cholerae
Entry authors: Nocek B, Wu R, Osipiuk J, Moy S, Kim Y, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
L-asparagine + H(2)O = L-aspartate + NH(3)
Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-192037 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
asparaginase Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 337 amino acids
Theoretical weight: 37.49 KDa
Source organism: Vibrio cholerae
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9KQK3 (Residues: 1-337; Coverage: 100%)
Gene name: VC_1995
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand ACT 4 x ACT
Ligand GOL 1 x GOL
1 modified residue:
Ligand MSE 64 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P21
Unit cell:
a: 53.235Å b: 117.838Å c: 121.431Å
α: 90° β: 91.44° γ: 90°
R-values:
R R work R free
0.179 0.176 0.246
Expression system: Escherichia coli BL21(DE3)

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