› 2oeh

Solution NMR

Determination of the Three-dimensional Structure of the Mrf2-DNA Complex Using Paramagnetic Spin Labeling

Released:
DOI: 10.2210/pdb2oeh/pdb
PDB entry 2oeh coloured by chain, ensemble of 17 models, front view.
PDB entry 2oeh coloured by chain, ensemble of 17 models, side view.
PDB entry 2oeh coloured by chain, ensemble of 17 models, top view.
Source organism: Homo sapiens
Primary publication:
Determination of the three-dimensional structure of the Mrf2-DNA complex using paramagnetic spin labeling.
Cai S, Zhu L, Zhang Z, Chen Y
Biochemistry 46 4943-50 (2007)
PMID: 17407261

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero trimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115729 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
AT-rich interactive domain-containing protein 5B Chain: A
Molecule details ›
Chain: A
Length: 107 amino acids
Theoretical weight: 12.65 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli
UniProt:
  • Canonical: Q14865 (Residues: 318-424; Coverage: 9%)
Gene names: ARID5B, DESRT, MRF2
Sequence domains: ARID/BRIGHT DNA binding domain
Structure domains: ARID DNA-binding domain
5'-D(P*TP*AP*CP*AP*AP*TP*AP*TP*AP*AP*CP*GP*TP*CP*G)-3' Chain: B
Molecule details ›
Chain: B
Length: 15 nucleotides
Theoretical weight: 4.58 KDa
Source organism: Homo sapiens
Expression system: Not provided
5'-D(P*CP*GP*AP*CP*GP*TP*TP*AP*TP*AP*TP*TP*GP*T)-3' Chain: C
Molecule details ›
Chain: C
Length: 14 nucleotides
Theoretical weight: 4.29 KDa
Source organism: Homo sapiens
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: distance geometry simulated annealing
Expression systems:
  • Escherichia coli
  • Not provided

Quick links

Citations

9 review citations

Theory, practice, and applications of paramagnetic relaxation enhancement for the characterization of transient low-population states of biological macromolecules and their complexes.
Clore et al. (2009)
8 more

3 mentions without citation

The dipeptidyl peptidase IV inhibitors vildagliptin and K-579 inhibit a phospholipase C: a case of promiscuous scaffolds in proteins.
Chakraborty et al. (2013)
2 more