› 2ofq

Solution NMR

NMR Solution Structure of a complex between the VirB9/VirB7 interaction domains of the pKM101 type IV secretion system

Released:
DOI: 10.2210/pdb2ofq/pdb
PDB entry 2ofq coloured by chain, ensemble of 20 models, front view.
PDB entry 2ofq coloured by chain, ensemble of 20 models, side view.
PDB entry 2ofq coloured by chain, ensemble of 20 models, top view.
Source organism: IncN plasmid R46
Primary publication:
NMR structure of a complex between the VirB9/VirB7 interaction domains of the pKM101 type IV secretion system.
Bayliss R, Harris R, Coutte L, Monier A, Fronzes R, Christie PJ, Driscoll PC, Waksman G
Proc Natl Acad Sci U S A 104 1673-8 (2007)
PMID: 17244707

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-181284 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
TraO Chain: A
Molecule details ›
Chain: A
Length: 95 amino acids
Theoretical weight: 10.95 KDa
Source organism: IncN plasmid R46
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q79SE3 (Residues: 177-271; Coverage: 35%)
Gene name: traO
Sequence domains: Conjugal transfer protein
Structure domains: Immunoglobulin-like
TraN Chain: B
Molecule details ›
Chain: B
Length: 22 amino acids
Theoretical weight: 2.43 KDa
Source organism: IncN plasmid R46
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q79SE5 (Residues: 21-42; Coverage: 46%)
Gene name: traN
Sequence domains: P-type Type IV secretion system, TraN

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
Refinement method: simulated annealing
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

17 review citations

Biological diversity of prokaryotic type IV secretion systems.
Alvarez-Martinez et al. (2009)
16 more

1 mention without citation

NMR structure of a complex between the VirB9/VirB7 interaction domains of the pKM101 type IV secretion system.
Bayliss et al. (2007)