› 2og0

X-ray diffraction
1.9Å resolution

Crystal Structure of the Lambda Xis-DNA complex

Released:
DOI: 10.2210/pdb2og0/pdb
PDB entry 2og0 coloured by chain, front view.
PDB entry 2og0 coloured by chain, side view.
PDB entry 2og0 coloured by chain, top view.
Source organism: Lambdavirus lambda
Primary publication:
Fis targets assembly of the Xis nucleoprotein filament to promote excisive recombination by phage lambda.
Papagiannis CV, Sam MD, Abbani MA, Yoo D, Cascio D, Clubb RT, Johnson RC
J Mol Biol 367 328-43 (2007)
PMID: 17275024

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-115732 (preferred)
Entry contents:
1 distinct polypeptide molecule
2 distinct DNA molecules
Macromolecules (3 distinct):
Excisionase Chains: A, B
Molecule details ›
Chains: A, B
Length: 52 amino acids
Theoretical weight: 6.43 KDa
Source organism: Escherichia virus Lambda
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P03699 (Residues: 1-52; Coverage: 72%)
Gene name: xis
Sequence domains: Excisionase-like protein
Structure domains: Multidrug-efflux Transporter Regulator; Chain: A; Domain 2
5'-D(*GP*TP*AP*TP*TP*AP*TP*GP*TP*AP*GP*TP*CP*TP*GP*TP*TP*T)-3' Chain: C
Molecule details ›
Chain: C
Length: 18 nucleotides
Theoretical weight: 5.54 KDa
Source organism: Lambdavirus lambda
Expression system: Not provided
5'-D(*AP*AP*AP*CP*AP*GP*AP*CP*TP*AP*CP*AP*TP*AP*AP*TP*AP*C)-3' Chain: D
Molecule details ›
Chain: D
Length: 18 nucleotides
Theoretical weight: 5.49 KDa
Source organism: Lambdavirus lambda
Expression system: Not provided

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU RU200
Spacegroup: R3
Unit cell:
a: 57.483Å b: 57.483Å c: 163.613Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.194 0.191 0.248
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

3 review citations

The λ Integrase Site-specific Recombination Pathway.
Landy A. (2015)
2 more

4 mentions without citation

The protein interaction map of bacteriophage lambda.
Rajagopala et al. (2011)
3 more