› 2og4

X-ray diffraction
2Å resolution

Structure of an expanded Jab1-MPN-like domain of splicing factor Prp8p from yeast

Released:
DOI: 10.2210/pdb2og4/pdb
PDB entry 2og4 coloured by chain, front view.
PDB entry 2og4 coloured by chain, side view.
PDB entry 2og4 coloured by chain, top view.
Source organism: Saccharomyces cerevisiae RM11-1a
Primary publication:
Structure of a multipartite protein-protein interaction domain in splicing factor prp8 and its link to retinitis pigmentosa.
Pena V, Liu S, Bujnicki JM, Lührmann R, Wahl MC
Mol Cell 25 615-24 (2007)
PMID: 17317632

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-152595 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Pre-mRNA-splicing factor 8 Chain: A
Molecule details ›
Chain: A
Length: 254 amino acids
Theoretical weight: 28.74 KDa
Source organism: Saccharomyces cerevisiae RM11-1a
Expression system: Escherichia coli
UniProt:
  • Canonical: P33334 (Residues: 2147-2397; Coverage: 10%)
Gene names: DBF3, DNA39, PRP8, RNA8, SLT21, USA2, YHR165C
Sequence domains: PROCT (NUC072) domain
Structure domains: Cytidine Deaminase, domain 2

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: MPG/DESY, HAMBURG BEAMLINE BW6
Spacegroup: P42212
Unit cell:
a: 78.465Å b: 78.465Å c: 122.812Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.204 0.202 0.241
Expression system: Escherichia coli

Quick links

Citations

31 review citations

The spliceosome: design principles of a dynamic RNP machine.
Wahl et al. (2009)
30 more

4 mentions without citation

Crystal structure of Prp8 reveals active site cavity of the spliceosome.
Galej et al. (2013)
3 more