› 2og7

X-ray diffraction
1.66Å resolution

Cystal structure of asparagine oxygenase in complex with Fe(II), 2S,3S-3-hydroxyasparagine and succinate

Released:
DOI: 10.2210/pdb2og7/pdb
PDB entry 2og7 coloured by chain, front view.
PDB entry 2og7 coloured by chain, side view.
PDB entry 2og7 coloured by chain, top view.
Source organism: Streptomyces coelicolor A3(2)
Primary publication:
Mechanistic and structural basis of stereospecific Cbeta-hydroxylation in calcium-dependent antibiotic, a daptomycin-type lipopeptide.
Strieker M, Kopp F, Mahlert C, Essen LO, Marahiel MA
ACS Chem Biol 2 187-96 (2007)
PMID: 17373765

Function and Biology Details

Reaction catalysed: 
L-asparagine + 2-oxoglutarate + O(2) = (2S,3S)-3-hydroxyasparagine + succinate + CO(2)
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-195583 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
L-asparagine oxygenase Chain: A
Molecule details ›
Chain: A
Length: 357 amino acids
Theoretical weight: 38.95 KDa
Source organism: Streptomyces coelicolor A3(2)
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q9Z4Z5 (Residues: 2-333; Coverage: 100%)
Gene names: SCE29.05c, SCO3236, asnO
Sequence domains: Taurine catabolism dioxygenase TauD, TfdA family
Structure domains: Clavaminate synthase-like

Ligands and Environments

3 bound ligands:
Ligand FE2 1 x FE2
Ligand SIN 1 x SIN
Ligand AHB 1 x AHB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: EMBL/DESY, HAMBURG BEAMLINE X13
Spacegroup: P3121
Unit cell:
a: 91.308Å b: 91.308Å c: 90.867Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.184 0.168 0.184
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

10 review citations

Nonribosomal Peptide Synthesis-Principles and Prospects.
Süssmuth et al. (2017)
9 more

5 mentions without citation

Recent examples of α-ketoglutarate-dependent mononuclear non-haem iron enzymes in natural product biosyntheses.
Gao et al. (2018)
4 more