› 2og9

X-ray diffraction
1.9Å resolution

Crystal Structure of mandelate racemase/muconate lactonizing enzyme from Polaromonas sp. JS666

Released:
DOI: 10.2210/pdb2og9/pdb
PDB entry 2og9 coloured by chain, front view.
PDB entry 2og9 coloured by chain, side view.
PDB entry 2og9 coloured by chain, top view.
Source organism: Polaromonas sp. JS666
Entry authors: Kumaran D, Burley SK, Swaminathan S, New York SGX Research Center for Structural Genomics (NYSGXRC)

Function and Biology Details

Reaction catalysed: 
Galactarate = (2R,3S)-2,3-dihydroxy-5-oxohexanedioate + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo octamer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-171477 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Mandelate racemase/muconate lactonizing enzyme C-terminal domain-containing protein Chains: A, B
Molecule details ›
Chains: A, B
Length: 393 amino acids
Theoretical weight: 43.46 KDa
Source organism: Polaromonas sp. JS666
Expression system: Escherichia coli
UniProt:
  • Canonical: Q12GE3 (Residues: 2-383; Coverage: 100%)
Gene name: Bpro_0435
Sequence domains:
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand CA 2 x CA
1 modified residue:
Ligand MSE 20 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: I4
Unit cell:
a: 133.416Å b: 133.416Å c: 87.434Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.203 0.203 0.23
Expression system: Escherichia coli

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