› 2ogi

X-ray diffraction
1.85Å resolution

Crystal structure of a putative metal dependent phosphohydrolase (sag1661) from streptococcus agalactiae serogroup v at 1.85 A resolution

Released:
DOI: 10.2210/pdb2ogi/pdb
PDB entry 2ogi coloured by chain, front view.
PDB entry 2ogi coloured by chain, side view.
PDB entry 2ogi coloured by chain, top view.
Source organism: Streptococcus agalactiae serogroup V
Entry author: Joint Center for Structural Genomics (JCSG)

Function and Biology Details

Reaction catalysed: 
P(1),P(4)-bis(5'-adenosyl) tetraphosphate + H(2)O = 2 ADP
Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184267 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Bis(5'-nucleosyl)-tetraphosphatase, symmetrical Chains: A, B
Molecule details ›
Chains: A, B
Length: 196 amino acids
Theoretical weight: 22.59 KDa
Source organism: Streptococcus agalactiae serogroup V
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8DY32 (Residues: 1-195; Coverage: 100%)
Gene name: SAG1661
Sequence domains: HD domain
Structure domains: Hypothetical protein af1432

Ligands and Environments

4 bound ligands:
Ligand FE 4 x FE
Ligand CL 3 x CL
Ligand GDP 2 x GDP
Ligand MES 1 x MES
1 modified residue:
Ligand MSE 8 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P21
Unit cell:
a: 65.841Å b: 52.535Å c: 70.022Å
α: 90° β: 114.29° γ: 90°
R-values:
R R work R free
0.174 0.172 0.229
Expression system: Escherichia coli

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