› 2ogu

X-ray diffraction
3.23Å resolution

Crystal structure of the isolated MthK RCK domain

Released:
DOI: 10.2210/pdb2ogu/pdb
PDB entry 2ogu coloured by chain, front view.
PDB entry 2ogu coloured by chain, side view.
PDB entry 2ogu coloured by chain, top view.
Source organism: Methanothermobacter thermautotrophicus str. Delta H
Primary publication:
Dynamic oligomeric conversions of the cytoplasmic RCK domains mediate MthK potassium channel activity.
Kuo MM, Baker KA, Wong L, Choe S
Proc Natl Acad Sci U S A 104 2151-6 (2007)
PMID: 17287352

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
homo dimer (preferred)
homo hexamer
Assembly name:
PDBe Complex ID:
PDB-CPX-127897 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Calcium-gated potassium channel MthK Chain: A
Molecule details ›
Chain: A
Length: 233 amino acids
Theoretical weight: 25.65 KDa
Source organism: Methanothermobacter thermautotrophicus str. Delta H
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O27564 (Residues: 107-336; Coverage: 69%)
Gene names: MTH_1520, mthK
Sequence domains:
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL1-5
Spacegroup: R32
Unit cell:
a: 165.89Å b: 165.89Å c: 82.22Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.235 0.232 0.299
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

2 review citations

Ion channels in microbes.
Martinac et al. (2008)
1 more

3 mentions without citation

KTN (RCK) domains regulate K+ channels and transporters by controlling the dimer-hinge conformation.
Roosild et al. (2009)
2 more