› 2oi9

X-ray diffraction
2.35Å resolution

Structure of the 2C/Ld/QL9 allogeneic complex

Released:
DOI: 10.2210/pdb2oi9/pdb
PDB entry 2oi9 coloured by chain, front view.
PDB entry 2oi9 coloured by chain, side view.
PDB entry 2oi9 coloured by chain, top view.
Source organism: Mus musculus
Primary publication:
How a single T cell receptor recognizes both self and foreign MHC.
Colf LA, Bankovich AJ, Hanick NA, Bowerman NA, Jones LL, Kranz DM, Garcia KC
Cell 129 135-46 (2007)
PMID: 17418792

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
hetero tetramer (preferred)
PDBe Complex ID:
PDB-CPX-106900 (preferred)
Entry contents:
4 distinct polypeptide molecules
Macromolecules (4 distinct):
H-2 class I histocompatibility antigen, L-D alpha chain Chain: A
Molecule details ›
Chain: A
Length: 179 amino acids
Theoretical weight: 20.94 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P01897 (Residues: 25-203; Coverage: 53%)
Gene name: H2-L
Sequence domains: Class I Histocompatibility antigen, domains alpha 1 and 2
Structure domains: MHC class I-like antigen recognition-like
T-cell receptor alpha chain V region PHDS58 Chain: B
Molecule details ›
Chain: B
Length: 113 amino acids
Theoretical weight: 12.49 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P01738 (Residues: 21-130; Coverage: 100%)
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins
Ig-like domain-containing protein Chain: C
Molecule details ›
Chain: C
Length: 121 amino acids
Theoretical weight: 13.27 KDa
Source organism: Mus musculus
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: A2NTY6 (Residues: 30-144; Coverage: 96%)
Sequence domains: Immunoglobulin V-set domain
Structure domains: Immunoglobulins
peptide (GLN)(LEU)(SER)(PRO)(PHE)(PRO)(PHE)(ASP)(LEU) Chain: Q
Molecule details ›
Chain: Q
Length: 9 amino acids
Theoretical weight: 1.06 KDa

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SSRL BEAMLINE BL11-1
Spacegroup: P6522
Unit cell:
a: 163.171Å b: 163.171Å c: 95.028Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.221 0.22 0.226
Expression system: Escherichia coli BL21

Quick links

Citations

55 review citations

T cell antigen receptor recognition of antigen-presenting molecules.
Rossjohn et al. (2015)
54 more

22 mentions without citation

The molecular basis of TCR germline bias for MHC is surprisingly simple.
Garcia et al. (2009)
21 more