› 2oiw

X-ray diffraction
2Å resolution

The structure of a predicted thioesterase from Bacillus stearothermophilus

Released:
DOI: 10.2210/pdb2oiw/pdb
PDB entry 2oiw coloured by chain, front view.
PDB entry 2oiw coloured by chain, side view.
PDB entry 2oiw coloured by chain, top view.
Source organism: Geobacillus stearothermophilus
Entry authors: Cuff ME, Li H, Moy S, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-177393 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Hypothetical conserved protein Chains: A, B, C, D
Molecule details ›
Chains: A, B, C, D
Length: 136 amino acids
Theoretical weight: 15.96 KDa
Source organism: Geobacillus stearothermophilus
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q5KYT1 (Residues: 1-133; Coverage: 100%)
Gene name: GK1870
Sequence domains: Thioesterase-like superfamily
Structure domains: Hotdog Thioesterase

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand EDO 3 x EDO
1 modified residue:
Ligand MSE 16 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P21212
Unit cell:
a: 76.521Å b: 93.051Å c: 75.844Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.178 0.176 0.233
Expression system: Escherichia coli BL21(DE3)

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