› 2ojr

X-ray diffraction
2.6Å resolution

Structure of ubiquitin solved by SAD using the Lanthanide-Binding Tag

Released:
DOI: 10.2210/pdb2ojr/pdb
PDB entry 2ojr coloured by chain, front view.
PDB entry 2ojr coloured by chain, side view.
PDB entry 2ojr coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Double-lanthanide-binding tags for macromolecular crystallographic structure determination.
Silvaggi NR, Martin LJ, Schwalbe H, Imperiali B, Allen KN
J Am Chem Soc 129 7114-20 (2007)
PMID: 17497863

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-143327 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Ubiquitin Chain: A
Molecule details ›
Chain: A
Length: 111 amino acids
Theoretical weight: 12.44 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P0CG48 (Residues: 608-684; Coverage: 11%)
Gene name: UBC
Sequence domains: Ubiquitin family
Structure domains:

Ligands and Environments

1 bound ligand:
Ligand TB 2 x TB
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: NSLS BEAMLINE X29A
Spacegroup: P3221
Unit cell:
a: 57.87Å b: 57.87Å c: 115.26Å
α: 90° β: 90° γ: 120°
R-values:
R R work R free
0.218 0.216 0.254
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

9 review citations

Application of metal coordination chemistry to explore and manipulate cell biology.
Haas et al. (2009)
8 more

3 mentions without citation

Structural and functional studies on the interaction of GspC and GspD in the type II secretion system.
Korotkov et al. (2011)
2 more