› 2okg

X-ray diffraction
1.65Å resolution

Structure of effector binding domain of central glycolytic gene regulator (CggR) from B. subtilis

Released:
DOI: 10.2210/pdb2okg/pdb
PDB entry 2okg coloured by chain, front view.
PDB entry 2okg coloured by chain, side view.
PDB entry 2okg coloured by chain, top view.
Source organism: Bacillus subtilis
Primary publication:
Crystal structures of the effector-binding domain of repressor Central glycolytic gene Regulator from Bacillus subtilis reveal ligand-induced structural changes upon binding of several glycolytic intermediates.
Rezácová P, Kozísek M, Moy SF, Sieglová I, Joachimiak A, Machius M, Otwinowski Z
Mol Microbiol 69 895-910 (2008)
PMID: 18554327

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:
Structure domain:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128441 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Central glycolytic genes regulator Chains: A, B
Molecule details ›
Chains: A, B
Length: 255 amino acids
Theoretical weight: 27.94 KDa
Source organism: Bacillus subtilis
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: O32253 (Residues: 89-340; Coverage: 74%)
Gene names: BSU33950, cggR, yvbQ
Sequence domains: Putative sugar-binding domain
Structure domains: Rossmann fold

Ligands and Environments

2 bound ligands:
Ligand CL 3 x CL
Ligand G3H 1 x G3H
1 modified residue:
Ligand MSE 16 x MSE

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-ID
Spacegroup: P212121
Unit cell:
a: 52.603Å b: 83.804Å c: 116.721Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.198 0.196 0.239
Expression system: Escherichia coli BL21(DE3)

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Citations

3 review citations

The emerging role of native mass spectrometry in characterizing the structure and dynamics of macromolecular complexes.
Boeri Erba et al. (2015)
2 more

5 mentions without citation

Crystal structures of the effector-binding domain of repressor Central glycolytic gene Regulator from Bacillus subtilis reveal ligand-induced structural changes upon binding of several glycolytic intermediates.
Rezácová et al. (2008)
4 more