› 2okt

X-ray diffraction
1.3Å resolution

Crystal structure of O-succinylbenzoic acid synthetase from Staphylococcus aureus, ligand-free form

Released:
DOI: 10.2210/pdb2okt/pdb
PDB entry 2okt coloured by chain, front view.
PDB entry 2okt coloured by chain, side view.
PDB entry 2okt coloured by chain, top view.
Source organism: Staphylococcus aureus subsp. aureus COL
Primary publication:
Loss of quaternary structure is associated with rapid sequence divergence in the OSBS family.
Odokonyero D, Sakai A, Patskovsky Y, Malashkevich VN, Fedorov AA, Bonanno JB, Fedorov EV, Toro R, Agarwal R, Wang C, Ozerova ND, Yew WS, Sauder JM, Swaminathan S, Burley SK, Almo SC, Glasner ME
Proc Natl Acad Sci U S A 111 8535-40 (2014)
PMID: 24872444

Function and Biology Details

Reaction catalysed: 
(1R,6R)-6-hydroxy-2-succinylcyclohexa-2,4-diene-1-carboxylate = 2-succinylbenzoate + H(2)O
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-101605 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
o-succinylbenzoate synthase Chain: A
Molecule details ›
Chain: A
Length: 342 amino acids
Theoretical weight: 39.1 KDa
Source organism: Staphylococcus aureus subsp. aureus COL
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: A0A0H2WWB5 (Residues: 2-333; Coverage: 100%)
Gene names: SACOL1843, menC
Sequence domains: Enolase C-terminal domain-like
Structure domains:

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 31-ID
Spacegroup: P21
Unit cell:
a: 72.7Å b: 40.324Å c: 59.223Å
α: 90° β: 92.98° γ: 90°
R-values:
R R work R free
0.139 0.138 0.181
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

3 review citations

Biosynthesis and applications of prenylquinones.
Kawamukai M. (2018)
2 more

5 mentions without citation

Fast and anisotropic flexibility-rigidity index for protein flexibility and fluctuation analysis.
Opron et al. (2014)
4 more