› 2okx

X-ray diffraction
1.9Å resolution

Crystal structure of GH78 family rhamnosidase of Bacillus SP. GL1 AT 1.9 A

Released:
DOI: 10.2210/pdb2okx/pdb
PDB entry 2okx coloured by chain, front view.
PDB entry 2okx coloured by chain, side view.
PDB entry 2okx coloured by chain, top view.
Source organism: Bacillus sp. GL1
Primary publication:
Crystal structure of glycoside hydrolase family 78 alpha-L-Rhamnosidase from Bacillus sp. GL1.
Cui Z, Maruyama Y, Mikami B, Hashimoto W, Murata K
J Mol Biol 374 384-98 (2007)
PMID: 17936784

Function and Biology Details

Reaction catalysed: 
Hydrolysis of terminal non-reducing alpha-L-rhamnose residues in alpha-L-rhamnosides
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assemblies composition:
monomeric (preferred)
homo dimer
Assembly name:
PDBe Complex ID:
PDB-CPX-188119 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
alpha-L-rhamnosidase Chains: A, B
Molecule details ›
Chains: A, B
Length: 956 amino acids
Theoretical weight: 106.15 KDa
Source organism: Bacillus sp. GL1
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q93RE7 (Residues: 1-956; Coverage: 100%)
Gene name: rhaB
Sequence domains:
Structure domains:

Ligands and Environments

2 bound ligands:
Ligand CA 4 x CA
Ligand GOL 43 x GOL
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: SPRING-8 BEAMLINE BL38B1
Spacegroup: P212121
Unit cell:
a: 97.744Å b: 119.985Å c: 207.352Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.182 0.182 0.214
Expression system: Escherichia coli BL21(DE3)

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Citations

2 review citations

Updates on naringinase: structural and biotechnological aspects.
Puri M. (2012)
1 more

9 mentions without citation

The structure of a Streptomyces avermitilis α-L-rhamnosidase reveals a novel carbohydrate-binding module CBM67 within the six-domain arrangement.
Fujimoto et al. (2013)
8 more