› 2olg

X-ray diffraction
1.7Å resolution

Crystal structure of the serine protease domain of prophenoloxidase activating factor-I in a zymogen form

Released:
DOI: 10.2210/pdb2olg/pdb
PDB entry 2olg coloured by chain, front view.
PDB entry 2olg coloured by chain, side view.
PDB entry 2olg coloured by chain, top view.
Source organism: Holotrichia diomphalia
Primary publication:
Crystal structure of the serine protease domain of prophenoloxidase activating factor-I.
Piao S, Kim S, Kim JH, Park JW, Lee BL, Ha NC
J Biol Chem 282 10783-91 (2007)
PMID: 17287215

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-132184 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Phenoloxidase-activating factor 1 heavy chain Chain: A
Molecule details ›
Chain: A
Length: 278 amino acids
Theoretical weight: 30.6 KDa
Source organism: Holotrichia diomphalia
Expression system: Spodoptera frugiperda
UniProt:
  • Canonical: O97366 (Residues: 88-365; Coverage: 81%)
Gene names: PPAF-I, PPAF1
Sequence domains: Trypsin
Structure domains: Trypsin-like serine proteases

Ligands and Environments

3 bound ligands:
Ligand NAG 1 x NAG
Ligand SO4 1 x SO4
Ligand CA 1 x CA
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: PAL/PLS BEAMLINE 4A
Spacegroup: P212121
Unit cell:
a: 38.229Å b: 53.304Å c: 116.643Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.208 0.204 0.245
Expression system: Spodoptera frugiperda

Quick links

Citations

2 review citations

The proPO-system: pros and cons for its role in invertebrate immunity.
Cerenius et al. (2008)
1 more

4 mentions without citation

The expanding diversity of serine hydrolases.
Botos et al. (2007)
3 more