› 2om2

X-ray diffraction
2.2Å resolution

Crystal Structure Of Human G[alpha]i1 Bound To The Goloco Motif Of Rgs14

Released:
DOI: 10.2210/pdb2om2/pdb
PDB entry 2om2 coloured by chain, front view.
PDB entry 2om2 coloured by chain, side view.
PDB entry 2om2 coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structure-based protocol for identifying mutations that enhance protein-protein binding affinities.
Sammond DW, Eletr ZM, Purbeck C, Kimple RJ, Siderovski DP, Kuhlman B
J Mol Biol 371 1392-404 (2007)
PMID: 17603074

Function and Biology Details

Biochemical function:
Biological process:
Cellular component:
Sequence domains:
Structure domains:

Structure analysis Details

Assembly composition:
hetero dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-128999 (preferred)
Entry contents:
2 distinct polypeptide molecules
Macromolecules (2 distinct):
Guanine nucleotide-binding protein G(i) subunit alpha-1 Chains: A, C
Molecule details ›
Chains: A, C
Length: 325 amino acids
Theoretical weight: 37.16 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21
UniProt:
  • Canonical: P63096 (Residues: 31-354; Coverage: 92%)
Gene name: GNAI1
Sequence domains: G-protein alpha subunit
Structure domains:
Regulator of G-protein signaling 14 Chains: B, D
Molecule details ›
Chains: B, D
Length: 36 amino acids
Theoretical weight: 4.09 KDa
Source organism: Homo sapiens
Expression system: Not provided
UniProt:
  • Canonical: O43566 (Residues: 497-532; Coverage: 6%)
Gene name: RGS14
Sequence domains: GoLoco motif

Ligands and Environments

2 bound ligands:
Ligand MG 2 x MG
Ligand GDP 2 x GDP
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 22-ID
Spacegroup: P2221
Unit cell:
a: 69.592Å b: 82.311Å c: 187.566Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.227 0.227 0.26
Expression systems:
  • Escherichia coli BL21
  • Not provided

Quick links

Citations

9 review citations

Computer-aided design of functional protein interactions.
Mandell et al. (2009)
8 more

12 mentions without citation

Structure-based protocol for identifying mutations that enhance protein-protein binding affinities.
Sammond et al. (2007)
11 more