› 2omk

X-ray diffraction
1.8Å resolution

Structure of the Bacteroides Thetaiotaomicron Thiamin Pyrophosphokinase

Released:
DOI: 10.2210/pdb2omk/pdb
PDB entry 2omk coloured by chain, front view.
PDB entry 2omk coloured by chain, side view.
PDB entry 2omk coloured by chain, top view.
Source organism: Bacteroides thetaiotaomicron VPI-5482
Entry authors: Vorontsov II, Minasov G, Shuvalova L, Abdullah J, Collart FR, Joachimiak A, Anderson WF, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Reaction catalysed: 
ATP + thiamine = AMP + thiamine diphosphate
Biochemical function:
Biological process:
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-183683 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Thiamin pyrophosphokinase-like, catalytic domain Chains: A, B
Molecule details ›
Chains: A, B
Length: 231 amino acids
Theoretical weight: 25.78 KDa
Source organism: Bacteroides thetaiotaomicron VPI-5482
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q8A545 (Residues: 1-207; Coverage: 100%)
Gene name: BT_2397
Sequence domains:
Structure domains: Thiamin pyrophosphokinase, catalytic domain

Ligands and Environments

No bound ligands
1 modified residue:
Ligand SNN 2 x SNN

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 5ID-B, APS BEAMLINE 21-ID-D
Spacegroup: P21
Unit cell:
a: 56.309Å b: 66.084Å c: 57.552Å
α: 90° β: 99.9° γ: 90°
R-values:
R R work R free
0.166 0.165 0.2
Expression system: Escherichia coli BL21(DE3)

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