2omo

X-ray diffraction
1.83Å resolution

Putative antibiotic biosynthesis monooxygenase from Nitrosomonas europaea

Released:
Source organism: Nitrosomonas europaea
Entry authors: Osipiuk J, Evdokimova E, Kagan O, Savchenko A, Edwards A, Joachimiak A, Midwest Center for Structural Genomics (MCSG)

Function and Biology Details

Biochemical function:
Biological process:
  • not assigned
Cellular component:

Structure analysis Details

Assembly composition:
homo dimer (preferred)
PDBe Complex ID:
PDB-CPX-182713 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
ABM domain-containing protein Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 124 amino acids
Theoretical weight: 14.52 KDa
Source organism: Nitrosomonas europaea
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: Q82WP3 (Residues: 1-100; Coverage: 100%)
Gene name: NE0621
Sequence domains: Antibiotic biosynthesis monooxygenase
Structure domains: Alpha-Beta Plaits

Ligands and Environments

No bound ligands
1 modified residue:

Experiments and Validation Details

Entry percentile scores
X-ray source: APS BEAMLINE 19-BM
Spacegroup: P212121
Unit cell:
a: 50.155Å b: 99.248Å c: 192.672Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.191 0.19 0.228
Expression system: Escherichia coli BL21(DE3)