› 2on8

X-ray diffraction
1.35Å resolution

Gbeta1 stabilization by in vitro evolution and computational design

Released:
DOI: 10.2210/pdb2on8/pdb
PDB entry 2on8 coloured by chain, front view.
PDB entry 2on8 coloured by chain, side view.
PDB entry 2on8 coloured by chain, top view.
Source organism: Streptococcus sp. G148
Primary publication:
Optimization of the gbeta1 domain by computational design and by in vitro evolution: structural and energetic basis of stabilization.
Wunderlich M, Max KE, Roske Y, Mueller U, Heinemann U, Schmid FX
J Mol Biol 373 775-84 (2007)
PMID: 17868696

Function and Biology Details

Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
Sequence domain:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-148791 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Immunoglobulin G-binding protein G Chain: A
Molecule details ›
Chain: A
Length: 56 amino acids
Theoretical weight: 6.31 KDa
Source organism: Streptococcus sp. G148
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P19909 (Residues: 373-427; Coverage: 10%)
Gene name: spg
Sequence domains: B domain
Structure domains: Ubiquitin-like (UB roll)

Ligands and Environments

No bound ligands
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: BESSY BEAMLINE 14.1
Spacegroup: P4
Unit cell:
a: 52.104Å b: 52.104Å c: 22.765Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.162 0.158 0.195
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

1 review citation

Lessons about protein stability from in vitro selections.
Schmid FX. (2011)
8 other articles

5 mentions without citation

The solvent component of macromolecular crystals.
Weichenberger et al. (2015)
4 more