› 2onp

X-ray diffraction
2Å resolution

Arg475Gln Mutant of Human Mitochondrial Aldehyde Dehydrogenase, complexed with NAD+

Released:
DOI: 10.2210/pdb2onp/pdb
PDB entry 2onp coloured by chain, front view.
PDB entry 2onp coloured by chain, side view.
PDB entry 2onp coloured by chain, top view.
Source organism: Homo sapiens
Primary publication:
Structural and functional consequences of coenzyme binding to the inactive asian variant of mitochondrial aldehyde dehydrogenase: roles of residues 475 and 487.
Larson HN, Zhou J, Chen Z, Stamler JS, Weiner H, Hurley TD
J Biol Chem 282 12940-50 (2007)
PMID: 17327228

Function and Biology Details

Reaction catalysed: 
An aldehyde + NAD(+) + H(2)O = a carboxylate + NADH
Biochemical function:
Biological process:
Cellular component:
Sequence domains:

Structure analysis Details

Assembly composition:
homo tetramer (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-138420 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
Aldehyde dehydrogenase, mitochondrial Chains: A, B, C, D, E, F, G, H
Molecule details ›
Chains: A, B, C, D, E, F, G, H
Length: 500 amino acids
Theoretical weight: 54.47 KDa
Source organism: Homo sapiens
Expression system: Escherichia coli BL21(DE3)
UniProt:
  • Canonical: P05091 (Residues: 18-517; Coverage: 97%)
Gene names: ALDH2, ALDM
Sequence domains: Aldehyde dehydrogenase family
Structure domains:

Ligands and Environments


Cofactor: Ligand NAD 8 x NAD
4 bound ligands:
Ligand MG 8 x MG
Ligand NA 8 x NA
Ligand EDO 39 x EDO
Ligand GAI 28 x GAI
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU, NSLS BEAMLINE X26C
Spacegroup: P212121
Unit cell:
a: 142.854Å b: 150.837Å c: 177.482Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.207 0.204 0.24
Expression system: Escherichia coli BL21(DE3)

Quick links

Citations

18 review citations

Non-P450 aldehyde oxidizing enzymes: the aldehyde dehydrogenase superfamily.
Marchitti et al. (2008)
17 more

2 mentions without citation

Structural and functional consequences of coenzyme binding to the inactive asian variant of mitochondrial aldehyde dehydrogenase: roles of residues 475 and 487.
Larson et al. (2007)
1 more