› 2onu

X-ray diffraction
2.38Å resolution

Plasmodium falciparum ubiquitin conjugating enzyme PF10_0330, putative homologue of human UBE2H

Released:
DOI: 10.2210/pdb2onu/pdb
PDB entry 2onu coloured by chain, front view.
PDB entry 2onu coloured by chain, side view.
PDB entry 2onu coloured by chain, top view.
Source organism: Plasmodium falciparum 3D7
Entry authors: Dong A, Lew J, Lin L, Hassanali A, Zhao Y, Senisterra G, Wasney G, Vedadi M, Kozieradzki I, Bochkarev A, Arrowsmith CH, Edwards AM, Sundstrom M, Weigelt J, Hui R, Brokx SJ, Structural Genomics Consortium (SGC)

Function and Biology Details

Reaction catalysed: 
S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine
Biochemical function:
  • not assigned
Biological process:
  • not assigned
Cellular component:
  • not assigned
Sequence domains:

Structure analysis Details

Assembly composition:
monomeric (preferred)
Assembly name:
PDBe Complex ID:
PDB-CPX-184793 (preferred)
Entry contents:
1 distinct polypeptide molecule
Macromolecule:
E2 ubiquitin-conjugating enzyme Chain: A
Molecule details ›
Chain: A
Length: 152 amino acids
Theoretical weight: 17.12 KDa
Source organism: Plasmodium falciparum 3D7
Expression system: Escherichia coli
UniProt:
  • Canonical: Q8IJ70 (Residues: 5-155; Coverage: 79%)
Gene name: PF3D7_1033900
Sequence domains: Ubiquitin-conjugating enzyme
Structure domains: Ubiquitin Conjugating Enzyme

Ligands and Environments

1 bound ligand:
Ligand PG4 1 x PG4
No modified residues

Experiments and Validation Details

Entry percentile scores
X-ray source: RIGAKU
Spacegroup: P43212
Unit cell:
a: 52.821Å b: 52.821Å c: 206.886Å
α: 90° β: 90° γ: 90°
R-values:
R R work R free
0.215 0.213 0.25
Expression system: Escherichia coli

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